Congratulations to the ChEMTox lab at Universidad Metropolitana, directed by Dr. Beatriz Zayas, for publishing their evaluation of nitrobenzazolo [3,2-a]quinolinium salts as potential anticancer agents!
To assess apoptosis, the activation of caspases 3 & 7 in the A431 epidermoid carcinoma cells was monitored using the ImmunoChemistry Technologies Magic Red™ Caspase 3&7 Assay. Cells stained with this assay that contain activated caspases 3 & 7 will exhibit a red fluorescence that may be monitored via fluorescence detection methods. This substrate-based caspase assay does not require a wash step, permeabilization, or fixation.