Thursday, July 30, 2009

Six New Citations using ICT's Kits

Biological Significance of Prolactin in Gynecologic Cancers
Vera V. Levina, Brian Nolen, YunYun Su, Andrew K. Godwin, David Fishman, Jinsong Liu, Gil Mor, Larry G. Maxwell, Ronald B. Herberman, Miroslaw J. Szczepanski, Marta E. Szajnik, Elieser Gorelik, and Anna E. Lokshin
Cancer Res., Jun 2009; 69: 5226 – 5233
...and by analysis of caspase activation using Fluorescent Poly-Caspases FLICA Apoptosis Detection kit FAM-VAD-FMK (Immunochemistry Technologies) as previously described (17). Multiplex analysis of phosphoproteins. Analysis of phosphoproteins was performed... ...

Culling of Activated CD4 T Cells during Typhoid Is Driven by Salmonella Virulence Genes
Aparna Srinivasan, Minelva Nanton, Amanda Griffin, and Stephen J. McSorley
J. Immunol., Jun 2009; 182: 7838 – 7845
...was detected by flow cytometry using the FLICA apoptosis detection kit according to the manufacturer's instructions (Immunochemistry Technologies). Results Depletion of activated Salmonella-specific T cells in mice infected with live bacteria Several...

Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis
Lan Shao, Hiroshi Fujii, Inés Colmegna, Hisashi Oishi, Jörg J. Goronzy, and Cornelia M. Weyand
J. Exp. Med., Jun 2009; 206: 1435 – 1449
...Particles to quantify cell numbers. Cell apoptosis was detected by caspases 3 and 7 SR FLICA kits (ImmunoChemistry Technologies), and rates of apoptotic cells were quantified through PI staining (Sigma-Aldrich). Comet assay. DNA damage was... ...

Farnesol-Induced Apoptosis in Candida albicans
Mark E. Shirtliff, Bastiaan P. Krom, Roelien A. M. Meijering, Brian M. Peters, Jingsong Zhu, Mark A. Scheper, Megan L. Harris, and Mary Ann Jabra-Rizk
Antimicrob. Agents Chemother., Jun 2009; 53: 2392 - 2401.
...mitochondrial permeability transition detection kit (Immunochemistry Technologies, LLC, Bloomington, MN) according to the manufacturers...microscopically using FLICA apoptosis detection kits (Immunochemistry Technologies, LLC) according to the manufacturer's recommendation... ...

Impairment of APE1 Function Enhances Cellular Sensitivity to Clinically Relevant Alkylators and Antimetabolites
Daniel R. McNeill, Wing Lam, Theodore L. DeWeese, Yung-Chi Cheng, and David M. Wilson, III
Mol. Cancer Res., Jun 2009; 7: 897 – 906
...red fluorescence above 600 nm. Apoptosis was measured using the poly-caspases FLICA apoptosis detection kit from Immunochemistry Technologies, LLC. The kit uses an inhibitor sequence of caspases (VAD, which reacts with all caspases) linked to a green (carboxyfluorescein... ...

Phenotypic and Functional Changes Induced in Hematopoietic Stem/Progenitor Cells After Gamma-Ray Radiation ExposureArthur J. Simonnet, Johnny Nehmé, Pierre Vaigot, Vilma Barroca, Philippe Leboulch, and Diana Tronik-Le Roux
Stem Cells, Jun 2009; 27: 1400 - 1409
...for another 15 minutes at 37C and immediately analyzed by flow cytometry. Apoptosis Assay A caspase 3/7 FLICA Kit, Immunochemistry Technologies, LLC, Bloomington, MN, http:www.immunochemistry.com, was used according to the manufacturer's protocol...

Wednesday, April 8, 2009

Welcome to ICT's Blog

Based in Bloomington, Minnesota, ICT is one of the world's leading suppliers of unique in vivo and in vitro tools critical for discovering promising investigational medicines for treating life threatening illnesses.

Our fluorescent enzyme detection probes are used to assess apoptosis and cell death in cultured cells. ICT's specialty in vitro probes detect caspases, cathepsins, serine proteases, cholinesterase enzymes, and assess mitochondrial health. We also manufacture proprietary probes that can detect dead and dying cells in living organisms in vivo for research use. In the future, these probes may be used to better manage disease and personalize treatment. ICT's in vivo probes may be used to assess the efficacy of chemotherapy, to quantify neurodegeneration, and as an early detector of eye disease, among other applications.

ICT is also a contract research laboratory specializing in custom ELISA development, manufacturing, and testing services. As an out-growth of these services, ICT offers a specialty line of ELISA buffers and stabilizers to researchers developing in-house assays.