Recent Publications Highlight the Use of FLIVO to Detect Apoptotic Dendritic Cells in vivo

CCR7-Dependent Stimulation of Survival in Dendritic Cells Involves Inhibition of GSK3β
Cristina Escribano, Cristina Delgado-Martín, and José Luis Rodríguez-Fernández. J. Immunol., Nov 2009; 183: 6282 – 6295.
...Sulforhodamine B (SR)-FLIVO (SR, abs 565 nm, em 600 nm), a form of FLIVO conjugated to sulforhodamine B, was obtained from Immunochemistry Technologies... SR-FLIVO, an agent that selectively stains apoptotic (cells) in vivo...

Recent Magic Red and FLICA Citations

Magic Red
Xiang Shen, Jeong-Seok Park, Ye Qiu, Joel Sugar, and Beatrice Y J T Yue. Effects of Sp1 overexpression on cultured human corneal stromal cells. Genes Cells, Oct 2009; 14: 1133 – 1139.
...Cathepsin B activity The activity of cathepsin B in transfected cells was detected using the Magic Red cathepsin B kit (Immunochemistry Technologies, Bloomington, MN, USA). Ki-67 staining Transfected cells were fixed in cold methanol and permeabilized. After...

FLICA
Soumika Biswas, Kaushik Biswas, Amy Richmond, Jennifer Ko, Sankar Ghosh, Matthew Simmons, Patricia Rayman, Brian Rini, Inderbir Gill, Charles S. Tannenbaum, and James H. Finke. Elevated Levels of Select Gangliosides in T Cells from Renal Cell Carcinoma Patients Is Associated with T Cell Dysfunction. J. Immunol., Oct 2009; 183: 5050–5058.
...and GM2. To study activation of caspases, cells were stained with fluorochrome inhibitors of caspases (FLICA) from Immunochemistry Technologies for 1 h at 37C protected from light, followed by staining with hamster anti-human GM2 Ab. Induction of ROS was determined...

S. Grunewald, C. Kriegel, T. Baumann, H.-J. Glander, and U. Paasch. Interactions between apoptotic signal transduction and capacitation in human spermatozoa. Hum. Reprod., Sep 2009; 24: 2071–2078.
...the appropriate controls according to the kit instructions provided by the manufacturer (Carboxyfluorescein FLICA, Immunochemistry Technologies, Bloomington, MN, USA). A 150-fold stock solution of the inhibitor was prepared in DMSO and further diluted in phosphate...

FAM-FLICA Caspase-1
Unja Martin, John Scholler, Jesse Gurgel, Blair Renshaw, John E. Sims, and Christopher A. Gabel. Externalization of the Leaderless Cytokine IL-1F6 Occurs in Response to Lipopolysaccharide/ATP Activation of Transduced Bone Marrow Macrophages. J. Immunol., Sep 2009; 183: 4021–4030.
...anti-GFP mouse monoclonal from Clontech, and anti-His mouse monoclonal from Roche. Caspase-1 FLICA kit was from Immunochemistry Technologies. Recombinant mcsf-1 and CP-456773 were generated at Amgen. Isolation of murine macrophages C57BL/6 mice were...

Study describes role of Caspase-1 in infection of Chlamydia Trachomatis, use of FAM-YVAD-FMK cited

In the well-crafted study of inflammasome induction following cervical epithelial cell infection, Abdul-Sater et. al. (Inflammasome-Dependent Caspase-1 Activation in Cervical Epithelial Cells Stimulates Growth of the Intracellular Pathogen Chlamydia Trachomatis, J. Biol. Chem 284: 26789-26796, 2009) used ImmunoChemistry Technologies’ FLICA caspase-1 assay kit (catalog #98, FAM-YVAD-FMK) to quantify the percentage of cells containing active caspase-1 following C. Trachomatis infection of HeLa cells. The data show a significant level of caspase-1 activation within 24 hours post-infection evident in 50% of the cultured epithelial cells. The study goes further to illustrate the role of ROS production and K+ efflux in the activation of caspase-1. Specifically, infection by C. Trachomatis leads to a decrease in intracellular K+ levels that causes a rise in ROS production resulting in caspase-1 activation. Furthermore, the study illustrates how subsequent caspase-1 activation after C. Trachomatis infection enhances the infectivity of the pathogen.

Study Reveals Kinetics in Turnover of Young Rat Urothelium, Employing FAM-FLIVO™ for in vivo Apoptosis Detection

The recently published study by Erman et. al. (Apoptosis and Desquamation of Urothelial Cells in Tissue Remodeling During Rat Postnatal Development, J. Histochem. Cytochem. 57(8):721-730, 2009) used ImmunoChemistry Technologies’ FAM-FLIVO reagent (catalog #981) to observe the levels of apoptosis in urothelial cells of the young rat bladder. The study clearly demonstrates the kinetics in turnover of rat urothelium in the early postnatal period (birth to day 14) evidenced by apoptosis, autophagic cell death, and desquamation.

FLIVO and FLICA References

FAM-FLIVO™
Andreja Erman, Dasa Zupancic, and Kristijan Jezernik, Apoptosis and Desquamation of Urothelial Cells in Tissue Remodeling During Rat Postnatal Development. J. Histochem. Cytochem., Aug 2009; 57: 721 - 730.

FAM-FLICA™
Shira D. P. Rabin, Jared G. Flitton, and Donald R. Demuth, Aggregatibacter actinomycetemcomitans Cytolethal Distending Toxin Induces Apoptosis in Nonproliferating Macrophages by a Phosphatase-Independent Mechanism. Infect. Immun., Aug 2009; 77: 3161 - 3169.

ImmunoChemistry Technologies Receives Fast Track Grant Award from National Institutes of Health

Bloomington, Minn, September 11, 2009 – ImmunoChemistry Technologies, LLC, announced today that the National Cancer Institute (NCI) of the National Institutes of Health (NIH) has approved funding for Phase II of a fast track Phase I/II Small Business Innovation Research (SBIR) Grant, first awarded to the Company in September 2008. The award of over $640,000 is the second installment of the combined $850,000 grant to develop and test new reagents (FLIVO™) for fluorescence imaging of apoptotic tissues in various animal models of anti-cancer therapy. The work will be done in collaboration with the University of Minnesota.
Read more about our research and FLIVO™ on our website.

ImmunoChemistry Technologies Receives Funding from National Institutes of Health

Bloomington, Minn, September 11, 2009 – ImmunoChemistry Technologies, LLC, announced today that the National Institute of Mental Health (NIMH) of the National Institutes of Health (NIH) has awarded the Company its 2nd year, $250,000 grant under its Small Business Technology Transfer Research (STTR) program to develop new reagents for future medical imaging of apoptotic tissues. The work will be done in collaboration with Vanderbilt University in Nashville, TN.
Read more about our research and our existing FLIVO™ tracers on our website.