New publications employ use of green FAM FLICA caspase assays for apoptosis detection

Following a low pH and H2O2 treatment, keratoconus fibroblast cultures (right) exhibit higher levels of caspase-3 activity than normal cultures (left) via staining with FAM-FLICA caspase 3 assay (green caspase 3 inhibitor probe, FAM-DEVD-FMK). Data courtesy of Drs. Kenney and Luthra, University of California Irvine Medical Center.



Globular Adiponectin as a Complete Mesoangioblast Regulator: Role in Proliferation, Survival, Motility, and Skeletal Muscle Differentiation
Fiaschi T, Tedesco FS, Giannoni E, Diaz-Manera J, Parri M, Cossu G, and Chiarugi P. Mol. Biol. Cell, Mar 2010; 21: 848 - 859.
...carboxyfluorescein FLICA (fluorescent-labeled inhibitor of caspases) apoptosis detection kit was from ImmunoChemistry Technologies (Bloomington... ...cells undergoing apoptosis was assayed by...FLICA apoptosis detection kit according to...manufacturer's protocol... Briefly, nonpermeabilized D16 cells were washed...

The Endoplasmic Reticulum Stress-Inducible Protein, Herp, Is a Potential Triggering Antigen for Anti-DNA Response
Hirabayashi Y, Oka Y, Ikeda T, Fujii H, Ishii T, Sasaki T, and Harigae H. J. Immunol., Mar 2010; 184: 3276 – 3283.
...To detect apoptosis, caspase activity was visualized as a green color using an FAM FLICA poly-caspase assay kit (Immunochemistry Technologies, Bloomington, MN)…

CD56highCD16–CD62L– NK Cells Accumulate in Allergic Contact Dermatitis and Contribute to the Expression of Allergic Responses
Carbone T, Nasorri F, Pennino D, Eyerich K, Foerster S, Loredana Cifaldi L, Traidl-Hoffman C, Behrendt H, and Cavani A. J. Immunol., Jan 2010; 184: 1102 - 1110.
...prior to NK cell exposure. Induction of keratinocyte apoptosis was determined using the caspases 3 and 7 FLICA kit (ImmunoChemistry Technologies, Bloomington, MN) according to the manufacturers protocol, and evaluated by FACS. ELISA Supernatants of NK cells...