New Product Release: Far-Red FLICA Caspase-1 Assay

MINNEAPOLIS, MN - ICT is pleased to announce the release of a far-red fluorescence-emitting inhibitor probe for the in vitro detection of activated caspase-1 (ICE) enzyme.

FLICA^® 660 Caspase-1 Assay

Toss the lysate methods. Our new Far-Red FLICA™ 660 Caspase-1 Assay allows for the rapid and reliable detection of caspase-1 enzyme (ICE) in whole, living cells or tissue samples. Perform multiplexing studies with blue, green, or orange-labeled biomarkers via flow cytometry and fluorescence microscopy.

Caspase-1-positive THP-1 cells fluoresce red with
the inhibitor-based FLICA 660 Caspase-1 Probe
(Copyright 2012 ImmunoChemistry Technologies, LLC)  

• Far-red caspase-1 probe excites at 660 nm, emits at 690 nm
• Perform multiplexing studies with green-labeled biomarkers like GFP
• Whole cell assay leaves cellular system intact for accurate analysis
• Analyze via flow cytometry and fluorescence microscopy

View assay details and data: http://www.immunochemistry.com/products/flica-660-caspase-1-detection-assay-kit-far-red-fluorescence.html

FLICA^®: Fluorescent Inhibitors of Caspase Activity

The new far-red FLICA® Caspase-1 Assay employs the new far-red fluorescent caspase-1 inhibitor probe, FLICA^® 660-YVAD-FMK, to detect and label active caspase-1 enzymes in living cells. The new caspase assay joins its green fluorescent analog, FAM-FLICA Caspase-1 Assay, in ICT's FLICA^® line of fluorescent caspase assays for apoptosis and protease detection.

Order FLICA^® 660 Caspase-1 Assay (catalog # 9122) online or place an order with our customer service associates by calling 1-800-829-3194 or faxing 952-888-8988. Orders are confirmed and shipped within 1 business day and delivered with overnight service.

Activated THP-1 cells show caspase-1 (ICE) activity with 
the green fluorescent FAM-FLICA Caspase-1 Assay
the inhibitor-based FLICA 660 Caspase-1 Probe
(Copyright 2012 ImmunoChemistry Technologies, LLC)

• Originally identified as Interleukin -1β Converting Enzyme or ICE
• Cleaves IL-1 beta and IL-18 into active forms
• Creation of the inflammasome during host responses to pathogens leads to the activation of caspase-1
• Active caspase-1 plays an antimicrobial role during infection, leading to pyroptosis
• Inflammation-related disease models have illustrated a role for caspase-1 in asthma, rheumatoid arthritis, multiple sclerosis and other disorders

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