Congratulations to our customers at the Frederick National Laboratory for Cancer Research and the Universidad Metropolitana School of Environmental Affairs for publishing their recent study of the effects of imidazolium-based compounds on 60 human cancer cell lines in the September 2014 issue of MedChemComm.
To assess potential toxicity of the compounds, they measured apoptosis via caspase activation using our FLICA® (Fluorescent Labeled Inhibitors of Caspases) probes that bind covalently with active caspase effector enzymes.
Malhotra, S. V., Kumar, V., Velez, C., & Zayas, B. (2014). Imidazolium-derived ionic salts induce inhibition of cancerous cell growth through apoptosis. Med. Chem. Commun., 2014, 5, 1404-1409.
First published online 24 Jun 2014 {Abstract}
From abstract:To assess potential toxicity of the compounds, they measured apoptosis via caspase activation using our FLICA® (Fluorescent Labeled Inhibitors of Caspases) probes that bind covalently with active caspase effector enzymes.
Malhotra, S. V., Kumar, V., Velez, C., & Zayas, B. (2014). Imidazolium-derived ionic salts induce inhibition of cancerous cell growth through apoptosis. Med. Chem. Commun., 2014, 5, 1404-1409.
First published online 24 Jun 2014 {Abstract}
A study of the effects of imidazolium-based ionic liquids on 60 human cancer cell lines representing diverse histologies has identified four compounds which show potency at a nanomolar dose. ....experiments with A431 human epidermoid carcinoma cells suggest the activation of an apoptotic pathway, due to activity of the initiator caspase 8 and effector caspase 3.
Image © Royal Society of Chemistry 2014, Malhotra, Kumar, Velez, and Zayas
FLICA® Caspase Assays: Assess apoptosis and necrosis in cultured cells
- Sensitive and early indicator of the initiation of the apoptotic process
- Distinguish apoptosis from necrosis
- Flexible analysis methods
- Green and red probes available
- Detect poly caspase activity or individual active caspase enzymes
| Caspase Target | Fluorescent Label | Caspase Detection Assay | |||
| Poly Caspases; apoptosis | FAM (green) SR (red) 660 (far-red) | FAM-FLICA™ Poly Caspase Assay Kit SR-FLICA™ Poly Caspase Assay Kit FLICA® 660 Poly Caspase Assay Kit | |||
| Caspase 1 (ICE); pyroptosis, inflammation | FAM (green) 660 (far-red) | FAM-FLICA™ Caspase 1 Assay Kit FLICA™ 660 Caspase 1 Assay Kit | |||
| Caspase 2; regulator | FAM (green) | FAM-FLICA™ Caspase 2 Assay Kit | |||
| Caspase 3/7; effectors, apoptosis (DEVD-ases) | FAM (green) | FAM-FLICA™ Caspase 3/7 Assay Kit | |||
| Caspase 6; effector, apoptosis | FAM (green) | FAM-FLICA™ Caspase 6 Assay Kit | |||
| Caspase 8; initiator, extrinsic apoptosis pathway | FAM (green) | FAM-FLICA™ Caspase 8 Assay Kit | |||
| Caspase 9; initiator, intrinsic apoptosis pathway | FAM (green) SR (red) | FAM-FLICA™ Caspase 9 Assay Kit SR-FLICA™ Caspase 9 Assay Kit | |||
| Caspase 10; initiator, extrinsic apoptosis pathway | FAM (green) | FAM-FLICA™ Caspase 10 Assay Kit | |||
| Caspase 13; effector, apoptosis | FAM (green) | FAM-FLICA™ Caspase 13 Assay Kit |
See the full kit listing, data, and citations on www.immunochemistry.com.
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