Friday, July 19, 2013
Monday, May 6, 2013
Study on Anticancer Potential of Quinolinium Salts Cites Magic Red Caspase Assay
Congratulations to the ChEMTox lab at Universidad Metropolitana, directed by Dr. Beatriz Zayas, for publishing their evaluation of nitrobenzazolo [3,2-a]quinolinium salts as potential anticancer agents!
To assess apoptosis, the activation of caspases 3 & 7 in the A431 epidermoid carcinoma cells was monitored using the ImmunoChemistry Technologies Magic Red™ Caspase 3&7 Assay. Cells stained with this assay that contain activated caspases 3 & 7 will exhibit a red fluorescence that may be monitored via fluorescence detection methods. This substrate-based caspase assay does not require a wash step, permeabilization, or fixation.
To assess apoptosis, the activation of caspases 3 & 7 in the A431 epidermoid carcinoma cells was monitored using the ImmunoChemistry Technologies Magic Red™ Caspase 3&7 Assay. Cells stained with this assay that contain activated caspases 3 & 7 will exhibit a red fluorescence that may be monitored via fluorescence detection methods. This substrate-based caspase assay does not require a wash step, permeabilization, or fixation.
Friday, March 8, 2013
Several New Citations for FLICA Caspase Assays
The FLICA® fluorescent caspase assays for apoptosis detection from ImmunoChemistry Technologies continue to be routinely referenced in customers' journal publications. Here are just a few that have been released in recent weeks:
Measuring Apoptosis by Microscopy and Flow cytometry
CM Henry, E Hollville, SJ Martin
Methods, Feb 2013; DOI: http://dx.doi.org/10.1016/j.ymeth.2013.01.008. [Abstract, In Press, Uncorrected Proof]
... These probes are cell-permeable and non-cytotoxic. The current protocol employs the poly-caspase FLICA probe (FAM-VAD-fmk), available from Immunochemistry technologies {sic}, that irreversibly binds to all active apoptotic caspases. ...
Pro-inflammatory cytokine and caspase-1 responses to pattern recognition receptor activation of neutrophils and dendritic cells in Behcet's disease
Türe-Özdemir, Filiz, Aysın Tulunay, Mehmet Onur Elbaşı, İmren Tatlı, Anne-Marie Maurer, Gonca Mumcu, Haner Direskeneli, and Emel Ekşioğlu-Demiralp.
Rheumatology (2013). [Abstract]
... DCs and neutrophils (3 × 10 5 cells/ml) were incubated with FAM-YVAD-FMK probe by using a Caspase 1 FLICA assay kit (ImmunoChemistry Technologies, LLC, Bloomington, MN, USA) according to the manufacturer's instructions and activated caspase-1 was analysed by ...
Up-regulation of a death receptor renders antiviral T cells susceptible to NK cell–mediated deletion
Dimitra Peppa, Upkar S. Gill, Gary Reynolds, Nicholas J.W. Easom, Laura J. Pallett, Anna Schurich, Lorenzo Micco, Gaia Nebbia, Harsimran D. Singh, David H. Adams, Patrick T.F. Kennedy, and Mala K. Maini
J Exp Med, Jan 2013; 210: 99-114. [Abstract]
...flow cytometry using the carboxyfluorescein-FLICA apoptosis detection kit. Histograms represent early apoptotic (FLICA+7AAD) and late apoptotic (FLICA+7AAD...
Measuring Apoptosis by Microscopy and Flow cytometry
CM Henry, E Hollville, SJ Martin
Methods, Feb 2013; DOI: http://dx.doi.org/10.1016/j.ymeth.2013.01.008. [Abstract, In Press, Uncorrected Proof]
... These probes are cell-permeable and non-cytotoxic. The current protocol employs the poly-caspase FLICA probe (FAM-VAD-fmk), available from Immunochemistry technologies {sic}, that irreversibly binds to all active apoptotic caspases. ...
Pro-inflammatory cytokine and caspase-1 responses to pattern recognition receptor activation of neutrophils and dendritic cells in Behcet's disease
Türe-Özdemir, Filiz, Aysın Tulunay, Mehmet Onur Elbaşı, İmren Tatlı, Anne-Marie Maurer, Gonca Mumcu, Haner Direskeneli, and Emel Ekşioğlu-Demiralp.
Rheumatology (2013). [Abstract]
... DCs and neutrophils (3 × 10 5 cells/ml) were incubated with FAM-YVAD-FMK probe by using a Caspase 1 FLICA assay kit (ImmunoChemistry Technologies, LLC, Bloomington, MN, USA) according to the manufacturer's instructions and activated caspase-1 was analysed by ...
Up-regulation of a death receptor renders antiviral T cells susceptible to NK cell–mediated deletion
Dimitra Peppa, Upkar S. Gill, Gary Reynolds, Nicholas J.W. Easom, Laura J. Pallett, Anna Schurich, Lorenzo Micco, Gaia Nebbia, Harsimran D. Singh, David H. Adams, Patrick T.F. Kennedy, and Mala K. Maini
J Exp Med, Jan 2013; 210: 99-114. [Abstract]
...flow cytometry using the carboxyfluorescein-FLICA apoptosis detection kit. Histograms represent early apoptotic (FLICA+7AAD) and late apoptotic (FLICA+7AAD...
Thursday, February 14, 2013
Our Lab Valentines: FLICA 660 is red, Hoechst stain is blue...
Wishing our customers and colleagues a sweet Valentine's Day from ICT!
Observed on February 14 in the United States and other countries, St. Valentine's Day is now associated with the expression of romantic love and friendship (see Wikipedia for the whole story). Its history and traditions don't have much to do with immunoassays or apoptosis detection (obviously), but since those are the research focuses of ImmunoChemistry Technologies, we take the opportunity to show our appreciation for the individuals who keep our office and lab running.
Among others, here are a few Valentine's Day cards I designed to share with ICT staff today.
FLICA 660 is red
Hoechst stain is blue
chocolate is sweet
and so are you
-EMC
Observed on February 14 in the United States and other countries, St. Valentine's Day is now associated with the expression of romantic love and friendship (see Wikipedia for the whole story). Its history and traditions don't have much to do with immunoassays or apoptosis detection (obviously), but since those are the research focuses of ImmunoChemistry Technologies, we take the opportunity to show our appreciation for the individuals who keep our office and lab running.
Among others, here are a few Valentine's Day cards I designed to share with ICT staff today.
FLICA 660 is red
Hoechst stain is blue
chocolate is sweet
and so are you
-EMC
Friday, December 21, 2012
Year-End Thanks and Season's Greetings
We would like to take this time at year's end to thank our customers and colleagues for choosing ICT as your reagent supplier, laboratory service provider, or research collaborator in 2012.
We appreciate the importance of your research and look forward to providing you with our research tools for apoptosis and assay development in the year ahead.
Wishing you all the joys of the season and a happy and prosperous new year!
Apoptosis Detection | Caspase Assays | Assay Development | ELISA Blocking Buffers
©2012 ImmunoChemistry Technologies, LLC. All Rights Reserved. | Bright Minds, Bright Solutions™
Monday, November 12, 2012
ICT to Sponsor NIH Symposium 11/29: Dendritic Cells in Health and Disease - A Tribute to Ralph Steinman
MINNEAPOLIS, Minnesota (November 12, 2012) - ImmunoChemistry Technologies, LLC has announced their sponsorship of the NIH symposium, Dendritic Cells in Health and Disease - A Tribute to Ralph Steinman, scheduled for November 29, 2012 in Bethesda, MD.
Thursday, Nov. 29
8 AM - 5 PM
Natcher Auditorium
Natcher Building
NIH Bethesda
Description from the Foundation for the National Institutes of Health (FHIH):
This symposium is co-sponsored by the Cytokine Interest and Immunology Interest Groups at NIH to honor the career achievements and major scientific contributions of Dr. Ralph Steinman. Steinman's discovery of DCs in 1973 and his subsequent research
Wednesday, September 12, 2012
ICT Sponsors Yale Immunobiology Student Symposium
MINNEAPOLIS, Minnesota (September 12, 2012) - ImmunoChemistry Technologies, LLC is proud to be a lead sponsor of the Inaugural Graduate Student Symposium at the Yale University Department of Immunobiology, to be held September 13, 2012 in New Haven, CT.
The symposium will include presentations by leading immunobiologists. The event organizers welcome the attendance of graduate students, faculty, and post-doctoral fellows from the greater Yale University community and invite students from other universities to attend.
http://immunobiology.yale.edu/programs/student_symposium.aspx
Description from the Yale Department of Immunobiology:
The symposium will include presentations by leading immunobiologists. The event organizers welcome the attendance of graduate students, faculty, and post-doctoral fellows from the greater Yale University community and invite students from other universities to attend.
http://immunobiology.yale.edu/programs/student_symposium.aspx
Description from the Yale Department of Immunobiology:
This symposium serves as an opportunity for us to invite scientists on the cutting edge, to speak about current breakthroughs and the future of their field. This year,
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