New Products: Affipure Goat HRP-Anti-IgG Secondaries

Affipure Goat HRP-Anti-IgG Secondaries for all ELISA formats and Western blotting are now available from ICT:

• HRP Goat anti-Human IgG Fc
• HRP Goat anti-Rabbit IgG Fc
• HRP Goat anti-Mouse IgG Fc

ICT's new online home has launched!

On September 17, 2010, ICT launched a completely new design on our website, immunochemistry.com. We added new features, such as a document library, instant credit card processing for product orders, and in-depth information about our assay development and custom laboratory services.

New publications employ use of green FAM FLICA caspase assays for apoptosis detection

Following a low pH and H2O2 treatment, keratoconus fibroblast cultures (right) exhibit higher levels of caspase-3 activity than normal cultures (left) via staining with FAM-FLICA caspase 3 assay (green caspase 3 inhibitor probe, FAM-DEVD-FMK). Data courtesy of Drs. Kenney and Luthra, University of California Irvine Medical Center.

MitoPT JC-1 assay recently cited in multiple in vitro cancer therapeutic studies

A431 cells, treated with predetermined IC50 concentration of novel anticancer agents, fluoresce green and orange-red with MitoPT JC-1. Data courtesy of Zayas/ Carro, Universidad Metropolitana.

Recent Publications Highlight the Use of FLIVO to Detect Apoptotic Dendritic Cells in vivo

CCR7-Dependent Stimulation of Survival in Dendritic Cells Involves Inhibition of GSK3β
Cristina Escribano, Cristina Delgado-Martín, and José Luis Rodríguez-Fernández. J. Immunol., Nov 2009; 183: 6282 – 6295.
...Sulforhodamine B (SR)-FLIVO (SR, abs 565 nm, em 600 nm), a form of FLIVO conjugated to sulforhodamine B, was obtained from Immunochemistry Technologies... SR-FLIVO, an agent that selectively stains apoptotic (cells) in vivo...

Recent Magic Red and FLICA Citations

Magic Red
Xiang Shen, Jeong-Seok Park, Ye Qiu, Joel Sugar, and Beatrice Y J T Yue. Effects of Sp1 overexpression on cultured human corneal stromal cells. Genes Cells, Oct 2009; 14: 1133 – 1139.
...Cathepsin B activity The activity of cathepsin B in transfected cells was detected using the Magic Red cathepsin B kit (Immunochemistry Technologies, Bloomington, MN, USA). Ki-67 staining Transfected cells were fixed in cold methanol and permeabilized. After...

FLICA
Soumika Biswas, Kaushik Biswas, Amy Richmond, Jennifer Ko, Sankar Ghosh, Matthew Simmons, Patricia Rayman, Brian Rini, Inderbir Gill, Charles S. Tannenbaum, and James H. Finke. Elevated Levels of Select Gangliosides in T Cells from Renal Cell Carcinoma Patients Is Associated with T Cell Dysfunction. J. Immunol., Oct 2009; 183: 5050–5058.
...and GM2. To study activation of caspases, cells were stained with fluorochrome inhibitors of caspases (FLICA) from Immunochemistry Technologies for 1 h at 37C protected from light, followed by staining with hamster anti-human GM2 Ab. Induction of ROS was determined...

S. Grunewald, C. Kriegel, T. Baumann, H.-J. Glander, and U. Paasch. Interactions between apoptotic signal transduction and capacitation in human spermatozoa. Hum. Reprod., Sep 2009; 24: 2071–2078.
...the appropriate controls according to the kit instructions provided by the manufacturer (Carboxyfluorescein FLICA, Immunochemistry Technologies, Bloomington, MN, USA). A 150-fold stock solution of the inhibitor was prepared in DMSO and further diluted in phosphate...

FAM-FLICA Caspase-1
Unja Martin, John Scholler, Jesse Gurgel, Blair Renshaw, John E. Sims, and Christopher A. Gabel. Externalization of the Leaderless Cytokine IL-1F6 Occurs in Response to Lipopolysaccharide/ATP Activation of Transduced Bone Marrow Macrophages. J. Immunol., Sep 2009; 183: 4021–4030.
...anti-GFP mouse monoclonal from Clontech, and anti-His mouse monoclonal from Roche. Caspase-1 FLICA kit was from Immunochemistry Technologies. Recombinant mcsf-1 and CP-456773 were generated at Amgen. Isolation of murine macrophages C57BL/6 mice were...

Study describes role of Caspase-1 in infection of Chlamydia Trachomatis, use of FAM-YVAD-FMK cited

In the well-crafted study of inflammasome induction following cervical epithelial cell infection, Abdul-Sater et. al. (Inflammasome-Dependent Caspase-1 Activation in Cervical Epithelial Cells Stimulates Growth of the Intracellular Pathogen Chlamydia Trachomatis, J. Biol. Chem 284: 26789-26796, 2009) used ImmunoChemistry Technologies’ FLICA caspase-1 assay kit (catalog #98, FAM-YVAD-FMK) to quantify the percentage of cells containing active caspase-1 following C. Trachomatis infection of HeLa cells. The data show a significant level of caspase-1 activation within 24 hours post-infection evident in 50% of the cultured epithelial cells. The study goes further to illustrate the role of ROS production and K+ efflux in the activation of caspase-1. Specifically, infection by C. Trachomatis leads to a decrease in intracellular K+ levels that causes a rise in ROS production resulting in caspase-1 activation. Furthermore, the study illustrates how subsequent caspase-1 activation after C. Trachomatis infection enhances the infectivity of the pathogen.