Cutting Edge: NF-B Activating Pattern Recognition and Cytokine Receptors License NLRP3 Inflammasome Activation by Regulating NLRP3 Expression
Franz G. Bauernfeind, Gabor Horvath, Andrea Stutz, Emad S. Alnemri, Kelly MacDonald, David Speert, Teresa Fernandes-Alnemri, Jianghong Wu, Brian G. Monks, Katherine A. Fitzgerald, Veit Hornung, and Eicke Latz
J. Immunol., Jul 2009; 183: 787 - 791.
...the caspase-1 carboxyfluorescein-YVAD-fluoromethylketone FLICA (fluorescent labeled inhibitors of caspases) kit (Immunochemistry Technologies) was used to stain active caspase-1. CD14-positive cells were analyzed for FLICA positivity by flow cytometry. The...
The nucleolar RNA methyltransferase Misu (NSun2) is required for mitotic spindle stability
Shobbir Hussain, Sandra Blanco Benavente, Elisabete Nascimento, Ilaria Dragoni, Agata Kurowski, Astrid Gillich, Peter Humphreys, and Michaela Frye
J. Cell Biol., Jul 2009; 186: 27 - 40.
...fluorochrome inhibitor of caspases (FLICA) apoptosis detection kit was used as per the manufacturers instructions (Immunochemistry Technologies). FLICA probe, which covalently binds to activated caspases, was incubated with cells for 1 h, and cells were washed...
Sulforaphane targets pancreatic tumour-initiating cells by NF-B-induced antiapoptotic signalling
G Kallifatidis, V Rausch, B Baumann, A Apel, B M Beckermann, A Groth, J Mattern, Z Li, A Kolb, G Moldenhauer, P Altevogt, T Wirth, J Werner, P Schemmer, M W Büchler, A V Salnikov, and I Herr
Gut, Jul 2009; 58: 949 - 963.
...FAM-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-fluoromethylketone (FLICA) was used according to the manufacturers protocol (Immunochemistry Technologies, Bloomington, Minnesota, USA). FLICA binds covalently to specific active caspases (thus the fluorochrome accumulates...
ATP-induced osteoclast function: the formation of sealing-zone like structure and the secretion of lytic granules via microtubule-deacetylation under the control of Syk
Ryoichi Hazama, Xiujuan Qu, Kunio Yokoyama, Chisato Tanaka, Emi Kinoshita, Jinsong He, Shusuke Takahashi, Kaoru Tohyama, Hirohei Yamamura, and Yumi Tohyama
Genes Cells, Jul 2009; 14: 871 - 884.
...Osteolytic granules were labeled with LysoTracker Red (Invitrogen) and Magic Red cathepsin K detection kit (Immunochemistry Technologies, Bloomington, MN, USA). Cathepsin K detection kit was also used to quantify the released enzyme activity in the...
