Year-End Thanks and Season's Greetings

We would like to take this time at year's end to thank our customers and colleagues for choosing ICT as your reagent supplier, laboratory service provider, or research collaborator in 2012.

We appreciate the importance of your research and look forward to providing you with our research tools for apoptosis and assay development in the year ahead.

Wishing you all the joys of the season and a happy and prosperous new year!







Apoptosis Detection | Caspase Assays | Assay Development | ELISA Blocking Buffers

©2012 ImmunoChemistry Technologies, LLC. All Rights Reserved. | Bright Minds, Bright Solutions™

ICT to Sponsor NIH Symposium 11/29: Dendritic Cells in Health and Disease - A Tribute to Ralph Steinman

MINNEAPOLIS, Minnesota (November 12, 2012) - ImmunoChemistry Technologies, LLC has announced their sponsorship of the NIH symposium, Dendritic Cells in Health and Disease - A Tribute to Ralph Steinman, scheduled for November 29, 2012 in Bethesda, MD.

Thursday, Nov. 29
8 AM - 5 PM
Natcher Auditorium
Natcher Building
NIH Bethesda

Description from the Foundation for the National Institutes of Health (FHIH):

This symposium is co-sponsored by the Cytokine Interest and Immunology Interest Groups at NIH to honor the career achievements and major scientific contributions of Dr. Ralph Steinman. Steinman's discovery of DCs in 1973 and his subsequent research

ICT Sponsors Yale Immunobiology Student Symposium

MINNEAPOLIS, Minnesota (September 12, 2012) - ImmunoChemistry Technologies, LLC is proud to be a lead sponsor of the Inaugural Graduate Student Symposium at the Yale University Department of Immunobiology, to be held September 13, 2012 in New Haven, CT.

The symposium will include presentations by leading immunobiologists. The event organizers welcome the attendance of graduate students, faculty, and post-doctoral fellows from the greater Yale University community and invite students from other universities to attend.

http://immunobiology.yale.edu/programs/student_symposium.aspx

Description from the Yale Department of Immunobiology:

This symposium serves as an opportunity for us to invite scientists on the cutting edge, to speak about current breakthroughs and the future of their field. This year,

New Product Release: Far-Red FLICA Caspase-1 Assay

MINNEAPOLIS, MN - ICT is pleased to announce the release of a far-red fluorescence-emitting inhibitor probe for the in vitro detection of activated caspase-1 (ICE) enzyme.

FLICA^® 660 Caspase-1 Assay

Toss the lysate methods. Our new Far-Red FLICA™ 660 Caspase-1 Assay allows for the rapid and reliable detection of caspase-1 enzyme (ICE) in whole, living cells or tissue samples. Perform multiplexing studies with blue, green, or orange-labeled biomarkers via flow cytometry and fluorescence microscopy.

Caspase-1-positive THP-1 cells fluoresce red with
the inhibitor-based FLICA 660 Caspase-1 Probe
(Copyright 2012 ImmunoChemistry Technologies, LLC)  

• Far-red caspase-1 probe excites at 660 nm, emits at 690 nm
• Perform multiplexing studies with green-labeled biomarkers like GFP
• Whole cell assay leaves cellular system intact for accurate analysis
• Analyze via flow cytometry and fluorescence microscopy

View assay details and data: http://www.immunochemistry.com/products/flica-660-caspase-1-detection-assay-kit-far-red-fluorescence.html

Technical Tips for Measuring Caspase-1 with FLICA™

Caspase-1 (ICE) Detection

Caspase-1, originally identified as Interleukin -1β Converting Enzyme or ICE (1, 2), plays a role in processing a wide variety of proteins, most notably several cytokines (3-5) and enzymes within the glycolytic pathway (6). The creation of the inflammasome during host responses to pathogens leads to the activation of caspase-1 (7, 8) and the class of cell death known as pyroptosis. Inflammation-related disease models have illustrated a role for caspase-1 in asthma, rheumatoid arthritis, multiple sclerosis and other disorders (7, 8).

A popular method of detecting caspase-1 activation in living cell samples is the FLICA assay from ImmunoChemistry Technologies. These whole cell assays utilize fluorescent-labeled caspase-1 inhibitor probes to form covalent bonds with activated caspase-1 enzymes within living, intact cells. I have collected a short list of technical recommendations that we have shared over the years with scientists using this assay in their research. Please contact us with any questions!

Recent Citations for MitoPT and Antigen Coating Buffer

Our products were recently cited in several journals. The first two used our MitoPT JC-1 kits to assess mitochondrial membrane permeability.

Stijlemans B, Caljon G, Natesan SKA, Saerens D, Conrath K, Perez-Morga D, Skepper JN, Nikolaou A, Brys L, Pays E, Magez S, Field MC, De Baetselier P, Muyldermans S. (2011). High Affinity Nanobodies against the Trypanosome brucei VSG Are Potent Trypanolytic Agents that Block Endocytosis. PLoS Pathog. doi:10.1371/journal.ppat.1002072. [Full Text]

Rosado-Berrios CA, Velez C, Zayas B. (2011). Mitochondrial permeability and toxicity of diethylhexyl and monoethylexyl phthalates on TK6 human lymphoblasts cells. Toxicol in Vitro. doi:10.1016/j.tiv.2011.08.001. [Abstract]

An article in the Journal of Neuroimmunology used our Antigen Coating Buffer to dilute and coat the recombinant GRP94 protein.

Suzuki S, Utsugisawa K, Iwasa K, Satoh T, Nagane Y, Yoshikawa H, Kuwana M, Suzuki N. (2011). Autoimmunity to endoplasmic reticulum chaperone GRP94 myasthenia gravis. J. Neuroimmunol. doi:10.1016/j.jneuroim.2011.06.011. [Abstract]

Products Used:

• MitoPT JC-1 Assay Kit (ICT# 924, 911)
• Antigen Coating Buffer (ICT# 6246, 6247, 6248, 6249)