January 2015 Citations for FLICA Assay Kits

Two new citations using our FLICA Assay kits were published this month.
Identification of Aim2 as a Sensor for DNA Vaccines. J. Immunol. vol 194 no. 2 630-636. [Abstract]

"Samples were then stained with a caspase-1 FAM/FLICA kit according to the manufacturers [sic] instructions (ImmunoChemistry Technologies, Bloomington, MN)."

 Paster, W, Bruger, AM, Katsch, K, Gregoire, C, Roncagalli, R, Fu, G, Gascoigne, NRJ, Nika, K, Cohnen, A, Feller, SM, Simister, PC, Molder, KC, Cordoba, SP, Dushek, O, Malissen, B, Acuto, O. (2015). A THEMIS:SHP1 complex promotes T-cell survival. EMBO Journal 10.15252/embj.201387725[Full Text]

"A FAM‐FLICA detection probe was used to assess poly‐caspase activity by flow cytometry."

Products Used:

• FAM-FLICA Caspase 1 Assay Kit (ICT#97, 98)
• FAM-FLICA Poly-Caspase Assay Kit (ICT#91, 92)

Recent Customer Citations for Magic Red Cathepsin B Assay Kits

Li, K, Yang, L, Zhang, C, Niu, Y, Wei, L, Liu, J. (2014). HPS6 interacts with dynactin p150Glued to mediate retrograde trafficking and maturation of lysosomes. J. Cell Sci. 127, 4574-4588. [Abstract]

"we incubated cells with Magic Red cathepsin B (MR catB), a membrane permeable probe that fluoresces..."

Bain, JM, Louw, J, Lewis, LE, Okai, B, Walls, CA, Ballou, ER, Walker, LA, Reid, D, Munro, CA, Brown, AJP, Brown, GD, Gow, NAR, Erwig, LP. (2014). Candida albicans Hypha Formation and Mannan Masking of β-Glucan Inhibit Macrophage Phagosome Maturation. mBio. vol. 5 no. 6 e01874-14. [Full Text]

Abstract:

Candida albicans is a major life-threatening human fungal pathogen in the immunocompromised host. Host defense against systemic Candida infection relies heavily on the capacity of professional phagocytes of the innate immune system to ingest and destroy fungal cells. A number of pathogens, including C. albicans, have evolved mechanisms that attenuate the efficiency of phagosome-mediated inactivation, promoting their survival and replication within the host. Here we visualize host-pathogen interactions using live-cell imaging and show that viable, but not heat- or UV-killed C. albicans cells profoundly delay phagosome maturation in macrophage cell lines and primary macrophages. The ability of C. albicans to delay phagosome maturation is dependent on cell wall composition and fungal morphology. Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes. The loss of cell wall O-mannan leads to exposure of β-glucan in the inner cell wall, facilitating recognition by Dectin-1, which is associated with enhanced phagosome maturation.

Products Used:

• Magic Red Cathepsin B Assay Kit (ICT# 937, 938)