Friday, March 8, 2013

Several New Citations for FLICA Caspase Assays

The FLICA® fluorescent caspase assays for apoptosis detection from ImmunoChemistry Technologies continue to be routinely referenced in customers' journal publications. Here are just a few that have been released in recent weeks:


Measuring Apoptosis by Microscopy and Flow cytometry
CM Henry, E Hollville, SJ Martin
Methods, Feb 2013; DOI: http://dx.doi.org/10.1016/j.ymeth.2013.01.008. [Abstract, In Press, Uncorrected Proof]

... These probes are cell-permeable and non-cytotoxic. The current protocol employs the poly-caspase FLICA probe (FAM-VAD-fmk), available from Immunochemistry technologies {sic}, that irreversibly binds to all active apoptotic caspases. ...


Pro-inflammatory cytokine and caspase-1 responses to pattern recognition receptor activation of neutrophils and dendritic cells in Behcet's disease
Türe-Özdemir, Filiz, Aysın Tulunay, Mehmet Onur Elbaşı, İmren Tatlı, Anne-Marie Maurer, Gonca Mumcu, Haner Direskeneli, and Emel Ekşioğlu-Demiralp.
Rheumatology (2013). [Abstract]

... DCs and neutrophils (3 × 10 5 cells/ml) were incubated with FAM-YVAD-FMK probe by using a Caspase 1 FLICA assay kit (ImmunoChemistry Technologies, LLC, Bloomington, MN, USA) according to the manufacturer's instructions and activated caspase-1 was analysed by ...


Up-regulation of a death receptor renders antiviral T cells susceptible to NK cell–mediated deletion
Dimitra Peppa, Upkar S. Gill, Gary Reynolds, Nicholas J.W. Easom, Laura J. Pallett, Anna Schurich, Lorenzo Micco, Gaia Nebbia, Harsimran D. Singh, David H. Adams, Patrick T.F. Kennedy, and Mala K. Maini
J Exp Med, Jan 2013; 210: 99-114. [Abstract]

...flow cytometry using the carboxyfluorescein-FLICA apoptosis detection kit. Histograms represent early apoptotic (FLICA+7AAD) and late apoptotic (FLICA+7AAD...