Measuring Caspase-1 Activity

Caspase-1 (ICE)

• Originally identified as Interleukin -1β Converting Enzyme or ICE
• Cleaves IL-1 beta and IL-18 into active forms
• Creation of the inflammasome during host responses to pathogens leads to the activation of caspase-1
• Active caspase-1 plays an antimicrobial role during infection, leading to pyroptosis
• Inflammation-related disease models have illustrated a role for caspase-1 in asthma, rheumatoid arthritis, multiple sclerosis and other disorders

Activated THP-1 cells show caspase-1 (ICE) activity with the green fluorescent FAM-FLICA Caspase-1 Detection Assay

FAM-FLICA™ Caspase-1 Assay
This in vitro assay employs a fluorescent green inhibitor probe to detect active caspase-1 enzymes in living cells or tissue samples.
View assay details and data: http://www.immunochemistry.com/products/green-flica-caspase-1-detection-assay-kit.html


FAM-FLICA Caspase-1 Assay Kit



• Direct, fast labeling of active caspase-1

• Flexible analysis with standard fluorescence detection methods

• Green fluorescence allows for multiplexing with blue, orange, and red fluorescent dyes

Recent Citations

Our FLICA™ Caspase-1 Assay (catalog number 98, containing FAM-YVAD-FMK reagent) continues to be routinely referenced in customers' publications. Here are a few of the latest:

E. D. Murphey. Cecal Ligation and Puncture-Induced Impairment of Innate Immune Function Does Not Occur in the Absence of Caspase-1. J. Immunol. 2011; 187:905-910.

Mélanie Anne Hamon and Pascale Cossart. K+ efflux, is required for histone-H3 dephosphorylation by Listeria LLO and other pore forming toxins. Infect. Immun., Apr 2011; 10.1128/IAI.01243-10.

Norma Olivares-Zavaleta, Aaron Carmody, Ronald Messer, William M. Whitmire, and Harlan D. Caldwell. Chlamydia pneumoniae Inhibits Activated Human T Lymphocyte Proliferation by the Induction of Apoptotic and Pyroptotic Pathways. J. Immunol. 2011; 186:7120-7126.


Technical Tips for Measuring Caspase-1 with FLICA™

• Create positive controls by inducing caspase-1 with a known inducing agent.  Search publications for the best method of caspase-1 induction in your particular cell line.  For example, we have used PMA and LPS with THP-1 cells to create known positives. Email us for more information.
• Perform thorough wash steps with a centrifuge and, if necessary, a centrifuge plate adaptor.  Passive washing with adherent cells is also possible by repeating the following steps 3x: aspirate the cell culture media, replace it with fresh media, and wait 10 minutes. However, some caspase-positive cells may have lost adherence and could be loose in the media; for this reason, the aspirated media must be collected and spun down for inclusion with the final analysis.

Coming soon: Red FLICA™ Caspase-1 Reagents
Perform multiplexing studies with green-labeled biomarkers via flow cytometry, fluorescence plate reader, and fluorescence microscopy.
Join our mailing list to be informed when this new product becomes available!



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