Recent Publications Highlight the Use of FLIVO to Detect Apoptotic Dendritic Cells in vivo

CCR7-Dependent Stimulation of Survival in Dendritic Cells Involves Inhibition of GSK3β
Cristina Escribano, Cristina Delgado-Martín, and José Luis Rodríguez-Fernández. J. Immunol., Nov 2009; 183: 6282 – 6295.
...Sulforhodamine B (SR)-FLIVO (SR, abs 565 nm, em 600 nm), a form of FLIVO conjugated to sulforhodamine B, was obtained from Immunochemistry Technologies... SR-FLIVO, an agent that selectively stains apoptotic (cells) in vivo...


Immunological synapse formation inhibits, via NF-kappaB and FOXO1, the apoptosis of dendritic cells.
Riol-Blanco L, Delgado-Martín C, Sánchez-Sánchez N, Alonso-C LM, Gutiérrez-López MD, Del Hoyo GM, Navarro J, Sánchez-Madrid F, Cabañas C, Sánchez-Mateos P, Rodríguez-Fernández JL. Nat Immunol., 2009 Jul 10(7):753-60. Epub 2009 Jun 7.
... To identify apoptotic DCs inside the lymph node, we used FLIVO. ... mice were injected intravenously with sulforhodamine-conjugated FLIVO ... λem > 600 nm)—were obtained from Immunochemistry Technologies... Using FLIVO, we analyzed whether IS formation was able to protect DCs from becoming apoptotic in the lymph node (Fig. 3a)...

A protocol to detect apoptotic dendritic cells in murine lymph nodes using multiphoton microscopy.
Delgado-Martín C, Riol-Blanco L, Alonso-C L M, Rodríguez-Fernández J L. Nature Protocols. DOI: 10.1038/nprot.2009.133. June 2009.
... In non-apoptotic DCs, which lack caspases, FLIVO reagents are not retained and (diffuse) out. However, in apoptotic DCs they form covalent bonds with ... λem >600 nm) (Cat No 983)(Immunochemistry Technologies, LLC)... In studies to ascertain whether a treatment increases or reduces ... The method is also useful to analyse the apoptosis of T cells7 or any other cell type able to migrate ...