MitoPT JC-1 assay recently cited in multiple in vitro cancer therapeutic studies

A431 cells, treated with predetermined IC50 concentration of novel anticancer agents, fluoresce green and orange-red with MitoPT JC-1. Data courtesy of Zayas/ Carro, Universidad Metropolitana.





Anticancer Effects of Alpinia pricei Hayata Roots.
CL Hsu, YS Yu, GC Yen. J. Agric. Food Chem., Jan 2010, 58 (4), pp 2201–2208.
... Mitochondrial Membrane Potential (ΔΨ m ) Analysis… The mitochondrial membrane potential was determined using a MitoPT 100 test kit (Immunochemistry Technologies, LLC, Bloomington, MN). JC-1 is a cationic dye that exhibits ...

Anticancer Effects of Flavonoid Derivatives Isolated from Millettia reticulata Benth in SK-Hep-1 Human Hepatocellular Carcinoma Cells.
SC Fang, CL Hsu, HT Lin, GC Yen. J. Agric. Food Chem., Jan 2010, 58 (2), pp 814–820.
... About 1 × 10^4 events were acquired and analyzed using CELL Quest software. Mitochondrial Membrane Potential (ΔΨ m ) Analysis… Mitochondrial membrane potential was determined using the MitoPT 100 test kit (Immunochemistry Technologies, LLC). ...

Mechanisms of Apoptotic Effects Induced by Resveratrol, Dibenzoylmethane, and Their Analogues on Human Lung Carcinoma Cells.
CJ Weng, YT Yang, CT Ho, GC Yen. J. Agric. Food Chem., Jun 2009; 57 (12), pp 5235–5243.
... Collapse of Mitochondrial Membrane Potential (ΔΨm) by Resveratrol, MR-3, and HMDB… The mitochondrial permeability transition is an important step in the induction of cellular apoptosis. The loss of mitochondrial membrane potential is a hallmark for apoptosis... The mitochondrial permeability transition detection kit (MitoPT) was purchased from Immunochemistry Technologies…